TLR agonists activate HPV11 E7-pulsed DCs to promote a specific T cell response in a murine model

TLR agonists activate HPV11 E7-pulsed DCs to promote a specific T cell response in a murine model

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: Some TLR agonists may up-regulate the activation of dendritic cells caused by viral antigenic peptides and antigen-specific cytotoxic T lymphocytes, which are crucial in HPV vaccine development.We investigated the ability of three TLR agonists, imiquimod, PIC and CpG, to stimulate the maturation of murine BM-DCs loaded with HPV11E7 CTL epitopes, and the subsequent effect on HPV-specific T cell responses and tumour protection in a C57BL/6 mouse model.We found that TLR agonists, knowall.blog mostly PIC and imiquimod, stimulated the maturation of BM-DCs pulsed with HPV11E7 CTL epitope peptide.In combination with the epitope peptide, the TLR agonists CPG and PIC augmented epitope-specific Th1 cytokine hacklinkci.com production in vivo, while imiquimod and CPG, but not PIC, enhanced Th1 cytokine production in vitro.

However, we failed to observe in vivo CTL cytotoxicity and anti-tumour protection upon TLR ligation in our mouse model.Our results demonstrate that TLR agonists activate HPV11E7 CTL epitope pulsed BM-DCs to promote specific Th1 immunity in C57BL/6 mouse model, indicating the promise of TLR agonists as adjuvants for HPV epitope/DC-based multifaceted vaccines against HPV infections such as condyloma accuminatum.